Hotspot

ndhC-trnV-UAC

LSC · 52201-53136 · 936 bp

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Pi 0.0634
MarkerSeek score 81.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
936
variable_sites
55
pi
0.0634
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAGCACTTG AGAAGGTCTACGGTTCGAGT 39.1 50.0 57.4 57.8 1010-1041 bp 1.0 58.1
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAGCACT AGAAGGTCTACGGTTCGAGT 38.1 50.0 55.2 57.8 1010-1041 bp 1.0 56.7
ndhC-trnV-UAC_p3 GCCAAAATAGGAATAGCACTTGA AGAAGGTCTACGGTTCGAGT 39.1 50.0 57.2 57.8 1009-1040 bp 1.0 56.0
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAGCACTTG GAAGGTCTACGGTTCGAGTC 39.1 55.0 57.4 57.5 1009-1040 bp 1.0 55.3
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAGCACTT AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.0 57.8 1010-1041 bp 1.0 55.0