Hotspot

psaA-ycf3

LSC · 43541-44238 · 698 bp

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Pi 0.0583
MarkerSeek score 82.50
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
698
variable_sites
40
pi
0.0583
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 809-841 bp 1.0 82.6
psaA-ycf3_p2 AGAAATGACCGGGTTTAGCC TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 826-858 bp 1.0 82.4
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT AGCCGCTGAGTATTGGAAAC 50.0 50.0 57.9 58.3 872-904 bp 1.0 81.7
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT CAAGCCGCTGAGTATTGGAA 50.0 50.0 57.9 58.3 874-906 bp 1.0 81.7
psaA-ycf3_p5 AGAAATGACCGGGTTTAGCC AGCCGCTGAGTATTGGAAAC 50.0 50.0 57.9 58.3 889-921 bp 1.0 81.5