Hotspot

trnT-GGU-psbD

LSC · 32962-34352 · 1391 bp

Back to Cleome

Pi 0.0665
MarkerSeek score 83.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1391
variable_sites
86
pi
0.0665
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 1512-1545 bp 1.0 87.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA ACCTACAAAAACGAAGCGGT 50.0 45.0 58.1 58.0 1515-1548 bp 1.0 87.5
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA ACCTACAAAAACGAAGCGGT 50.0 45.0 58.3 58.0 1489-1522 bp 1.0 86.0
trnT-GGU-psbD_p4 GGCCCTTTTAACTCAGTGGT ACCTACAAAAACGAAGCGGT 50.0 45.0 57.7 58.0 1527-1560 bp 1.0 85.8
trnT-GGU-psbD_p5 TGGTAGAGTAACGCCATGGT ACCTACAAAAACGAAGCGGT 50.0 45.0 58.4 58.0 1511-1544 bp 1.0 84.7