Hotspot

trnC-GCA-petN

LSC · 28818-29518 · 701 bp

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Pi 0.054
MarkerSeek score 82.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
701
variable_sites
37
pi
0.054
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG CCATACTACGAGGGAGAGGG 50.0 60.0 58.1 58.1 802-811 bp 1.0 83.9
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG CCCATACTACGAGGGAGAGG 50.0 60.0 58.1 58.1 803-812 bp 1.0 83.9
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CCCCATACTACGAGGGAGAG 50.0 60.0 58.1 58.1 804-813 bp 1.0 83.9
trnC-GCA-petN_p4 TCTATCGTTTAGGCGGCATG CCATACTACGAGGGAGAGGG 50.0 60.0 57.9 58.1 855-864 bp 1.0 83.0
trnC-GCA-petN_p5 TCTATCGTTTAGGCGGCATG CCCATACTACGAGGGAGAGG 50.0 60.0 57.9 58.1 856-865 bp 1.0 83.0