Hotspot

trnK-UUU-rps16

LSC · 4413-5520 · 1108 bp

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Pi 0.11
MarkerSeek score 82.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1108
variable_sites
108
pi
0.11
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 1262-1332 bp 1.0 90.9
trnK-UUU-rps16_p2 TCAGGATCAGTCGTGGTCTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 1258-1328 bp 1.0 90.4
trnK-UUU-rps16_p3 TTCAGGATCAGTCGTGGTCT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 1259-1329 bp 1.0 90.4
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1400-1470 bp 1.0 90.2
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 1401-1471 bp 1.0 90.2