primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	58.0	58.0	0.0	7.6	0.040076	4413	5520	1262-1332	1.000000	90.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCAGGATCAGTCGTGGTCTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.1	58.0	0.0	7.6	0.099609	4413	5520	1258-1328	1.000000	90.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TTCAGGATCAGTCGTGGTCT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.1	58.0	0.0	7.6	0.099609	4413	5520	1259-1329	1.000000	90.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4413	5520	1400-1470	1.000000	90.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4413	5520	1401-1471	1.000000	90.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	0.0	16.9	0.491095	6666	7214	634-648	1.000000	84.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCCACAGCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.4	58.1	0.0	16.9	0.499524	6666	7214	635-649	1.000000	84.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GTCCTTCAATTCAAGTCGCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.586716	6666	7214	620-634	1.000000	83.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GCGTTGCTTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.603543	6666	7214	601-615	1.000000	83.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCCACAGCAGATCATGTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.4	58.1	0.0	16.9	0.710916	6666	7214	636-650	1.000000	82.3
trnS-GCU-trnT-CGU_p1	trnS-GCU-trnT-CGU	1	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCA	20	22	50.0	40.9	57.8	56.9	0.0	0.0	3.248264	8634	9284	719-953	1.000000	61.1
trnS-GCU-trnT-CGU_p2	trnS-GCU-trnT-CGU	2	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCA	20	22	50.0	40.9	58.2	56.9	2.0	0.0	3.287421	8634	9284	717-951	1.000000	60.8
trnS-GCU-trnT-CGU_p3	trnS-GCU-trnT-CGU	3	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCAC	20	23	50.0	43.5	57.8	58.2	0.0	0.0	3.383831	8634	9284	719-953	1.000000	60.0
trnS-GCU-trnT-CGU_p4	trnS-GCU-trnT-CGU	4	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCAC	20	23	50.0	43.5	58.2	58.2	2.0	0.0	3.422988	8634	9284	717-951	1.000000	59.7
trnS-GCU-trnT-CGU_p5	trnS-GCU-trnT-CGU	5	CCAACGCTTTAGTCCACTCA	AGGAACGAATCACACTTTTACCA	20	23	50.0	39.1	57.8	58.3	0.0	0.0	3.453494	8634	9284	720-954	1.000000	59.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	CCATACTACGAGGGAGAGGG	20	20	50.0	60.0	58.1	58.1	0.0	0.0	0.169711	28818	29518	802-811	1.000000	83.9
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	CCCATACTACGAGGGAGAGG	20	20	50.0	60.0	58.1	58.1	0.0	0.0	0.169711	28818	29518	803-812	1.000000	83.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	CCCCATACTACGAGGGAGAG	20	20	50.0	60.0	58.1	58.1	0.0	0.0	0.169711	28818	29518	804-813	1.000000	83.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	TCTATCGTTTAGGCGGCATG	CCATACTACGAGGGAGAGGG	20	20	50.0	60.0	57.9	58.1	13.1	0.0	0.236499	28818	29518	855-864	1.000000	83.0
trnC-GCA-petN_p5	trnC-GCA-petN	5	TCTATCGTTTAGGCGGCATG	CCCATACTACGAGGGAGAGG	20	20	50.0	60.0	57.9	58.1	13.1	0.0	0.236499	28818	29518	856-865	1.000000	83.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32126	32889	867-871	1.000000	84.0
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32126	32889	846-850	1.000000	84.0
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	32126	32889	846-850	1.000000	83.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	32126	32889	825-829	1.000000	83.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	32126	32889	843-847	1.000000	83.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.106399	32962	34352	1512-1545	1.000000	87.6
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.128554	32962	34352	1515-1548	1.000000	87.5
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.325138	32962	34352	1489-1522	1.000000	86.0
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GGCCCTTTTAACTCAGTGGT	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.330309	32962	34352	1527-1560	1.000000	85.8
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TGGTAGAGTAACGCCATGGT	ACCTACAAAAACGAAGCGGT	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.478408	32962	34352	1511-1544	1.000000	84.7
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	43541	44238	809-841	1.000000	82.6
psaA-ycf3_p2	psaA-ycf3	2	AGAAATGACCGGGTTTAGCC	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	6.3	0.0	0.315866	43541	44238	826-858	1.000000	82.4
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	43541	44238	872-904	1.000000	81.7
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.377504	43541	44238	874-906	1.000000	81.7
psaA-ycf3_p5	psaA-ycf3	5	AGAAATGACCGGGTTTAGCC	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	6.3	0.0	0.385211	43541	44238	889-921	1.000000	81.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCAAAATAGGAATAGCACTTG	AGAAGGTCTACGGTTCGAGT	23	20	39.1	50.0	57.4	57.8	0.0	0.0	3.778409	52201	53136	1010-1041	1.000000	58.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAAATAGGAATAGCACT	AGAAGGTCTACGGTTCGAGT	21	20	38.1	50.0	55.2	57.8	0.0	0.0	3.947731	52201	53136	1010-1041	1.000000	56.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GCCAAAATAGGAATAGCACTTGA	AGAAGGTCTACGGTTCGAGT	23	20	39.1	50.0	57.2	57.8	0.0	0.0	4.032396	52201	53136	1009-1040	1.000000	56.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCAAAATAGGAATAGCACTTG	GAAGGTCTACGGTTCGAGTC	23	20	39.1	55.0	57.4	57.5	0.0	0.0	4.122181	52201	53136	1009-1040	1.000000	55.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAAATAGGAATAGCACTT	AGAAGGTCTACGGTTCGAGT	22	20	36.4	50.0	56.0	57.8	0.0	0.0	4.166870	52201	53136	1010-1041	1.000000	55.0
ycf4-cemA_p1	ycf4-cemA	1	TTGGCTTACTTCTTGCGTGT	AGGGCAAAAAGACTATAGATGCA	20	23	45.0	39.1	58.0	57.9	0.0	6.8	3.154291	62569	63275	747-807	1.000000	64.0
ycf4-cemA_p2	ycf4-cemA	2	TTGGCTTACTTCTTGCGTGT	CAGGGCAAAAAGACTATAGATGC	20	23	45.0	43.5	58.0	57.6	0.0	6.8	3.468164	62569	63275	748-808	1.000000	61.5
ycf4-cemA_p3	ycf4-cemA	3	TTGGCTTACTTCTTGCGTGT	GGGCAAAAAGACTATAGATGCA	20	22	45.0	40.9	58.0	56.5	0.0	6.8	3.557274	62569	63275	746-806	1.000000	60.8
ycf4-cemA_p4	ycf4-cemA	4	TTGGCTTACTTCTTGCGTGT	AGGGCAAAAAGACTATAGATGC	20	22	45.0	40.9	58.0	56.2	0.0	6.8	3.833307	62569	63275	747-807	1.000000	58.6
ycf4-cemA_p5	ycf4-cemA	5	TTGGCTTACTTCTTGCGTGT	GGGCAAAAAGACTATAGATGCAA	20	23	45.0	39.1	58.0	57.2	0.0	6.8	3.873319	62569	63275	746-806	1.000000	58.3
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATATATCCGCGT	ACAGCCAATCCGAAATGTCA	20	20	50.0	45.0	58.0	57.8	4.9	0.0	0.232931	71716	72513	910-911	1.000000	83.1
rpl20-rps12_p2	rpl20-rps12	2	TCCGAGCTATATATCCGCGT	ACAAGACAGCCAATCCGAAA	20	20	50.0	45.0	58.0	57.7	4.9	0.0	0.304456	71716	72513	915-916	1.000000	82.5
rpl20-rps12_p3	rpl20-rps12	3	TCCGAGCTATATATCCGCGT	AGACAGCCAATCCGAAATGT	20	20	50.0	45.0	58.0	57.5	4.9	0.0	0.531003	71716	72513	912-913	1.000000	80.7
rpl20-rps12_p4	rpl20-rps12	4	TTTGTTCTACGTCTCCGAGC	ACAGCCAATCCGAAATGTCA	20	20	50.0	45.0	57.7	57.8	0.0	0.0	0.541737	71716	72513	923-924	1.000000	80.6
rpl20-rps12_p5	rpl20-rps12	5	GTAAGTCGTGAATGAGCCCC	ACAGCCAATCCGAAATGTCA	20	20	55.0	45.0	58.3	57.8	0.0	0.0	0.545676	71716	72513	971-972	1.000000	80.3
ycf1_p1	ycf1	1	AAGATTGAGTCCTGGGTCGA	CAGACAGACGAACAGGTTCA	20	20	50.0	50.0	58.1	57.5	0.0	12.2	0.570468	117085	117204	781-781	0.500000	55.4
ycf1_p2	ycf1	2	AAGATTGAGTCCTGGGTCGA	ACAGACAGACGAACAGGTTC	20	20	50.0	50.0	58.1	57.5	0.0	2.1	0.572183	117085	117204	782-782	0.500000	55.4
ycf1_p3	ycf1	3	GAAGGGTCTTGCCAATGACA	CAGACAGACGAACAGGTTCA	20	20	50.0	50.0	58.1	57.5	8.6	12.2	0.599353	117085	117204	695-695	0.500000	55.2
ycf1_p4	ycf1	4	GAAGGGTCTTGCCAATGACA	ACAGACAGACGAACAGGTTC	20	20	50.0	50.0	58.1	57.5	8.6	2.1	0.601068	117085	117204	696-696	0.500000	55.2
ycf1_p5	ycf1	5	GACGGGGTATAGGGGAAAGA	CAGACAGACGAACAGGTTCA	20	20	55.0	50.0	57.9	57.5	0.0	12.2	0.601651	117085	117204	849-849	0.500000	55.2
ndhH_p1	ndhH	1	GAAAACGTCAACGACTGCTG	ACTAATCGATTGGGCAGCAG	20	20	50.0	50.0	58.0	58.0	9.9	23.3	0.046147	118555	119736	1896-1896	0.500000	59.6
ndhH_p2	ndhH	2	GAAAACGTCAACGACTGCTG	CCGTCTGCTAGAGCTTGAAG	20	20	50.0	55.0	58.0	58.1	9.9	2.7	0.085270	118555	119736	1622-1622	0.500000	59.3
ndhH_p3	ndhH	3	GAAAACGTCAACGACTGCTG	CGTTGTTGTATCCCTGCAGA	20	20	50.0	50.0	58.0	57.9	9.9	17.4	0.098495	118555	119736	1568-1568	0.500000	59.2
ndhH_p4	ndhH	4	GAAAACGTCAACGACTGCTG	ACTGCGAATTTCTAAGCCGT	20	20	50.0	45.0	58.0	57.9	9.9	0.0	0.099951	118555	119736	2101-2101	0.500000	59.2
ndhH_p5	ndhH	5	GAAAACGTCAACGACTGCTG	CACGAATCGCACGTAGAGAT	20	20	50.0	50.0	58.0	57.9	9.9	1.2	0.111938	118555	119736	1951-1951	0.500000	59.1
ndhG_p1	ndhG	1	TCTGCCTGTTGTTGATTGGA	TCGATTGGTTGATGCGAGTT	20	20	45.0	45.0	57.3	57.9	0.0	0.0	0.748396	122984	123514	1792-1792	0.500000	54.0
ndhG_p2	ndhG	2	TCTGCCTGTTGTTGATTGGA	CGATTGGTTGATGCGAGTTG	20	20	45.0	50.0	57.3	57.8	0.0	0.0	0.818915	122984	123514	1791-1791	0.500000	53.4
ndhG_p3	ndhG	3	TCTGCCTGTTGTTGATTGGA	GCGGCTGCAATTGCTATAAC	20	20	45.0	50.0	57.3	58.2	0.0	29.4	0.896306	122984	123514	1718-1718	0.500000	52.8
ndhG_p4	ndhG	4	TCTGCCTGTTGTTGATTGGA	TCCAATAGTTGCTTCAGCGG	20	20	45.0	50.0	57.3	58.3	0.0	0.0	0.917251	122984	123514	1734-1734	0.500000	52.7
ndhG_p5	ndhG	5	TCTGCCTGTTGTTGATTGGA	AGCGGCTGCAATTGCTATAA	20	20	45.0	45.0	57.3	58.3	0.0	29.4	0.975951	122984	123514	1719-1719	0.500000	52.2
ndhD_p1	ndhD	1	TCTGCTCCAAGAACAGAGGA	CCATCGGCATTCTTTCAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058142	124784	126226	2294-2294	0.500000	59.5
ndhD_p2	ndhD	2	AAACAACTCGAAGCATGGGT	CCATCGGCATTCTTTCAGGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061430	124784	126226	2200-2200	0.500000	59.5
ndhD_p3	ndhD	3	GGCATGAAACAACTCGAAGC	CCATCGGCATTCTTTCAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.073583	124784	126226	2206-2206	0.500000	59.4
ndhD_p4	ndhD	4	AACTCGCATCAACCAATCGA	CCATCGGCATTCTTTCAGGA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.135992	124784	126226	2694-2694	0.500000	58.9
ndhD_p5	ndhD	5	TGCTTCTGCTCCAAGAACAG	CCATCGGCATTCTTTCAGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.154471	124784	126226	2298-2298	0.500000	58.8
ndhF_p1	ndhF	1	CCATTTAGGTTTGAAATAGCGTGA	CCACCGAAGAAGCTCCTTTT	24	20	37.5	50.0	57.8	58.1	0.0	0.0	4.292117	129706	131973	2551-2551	0.500000	25.7
ndhF_p2	ndhF	2	TCCATTTAGGTTTGAAATAGCGTG	CCACCGAAGAAGCTCCTTTT	24	20	37.5	50.0	57.8	58.1	0.0	0.0	4.292117	129706	131973	2552-2552	0.500000	25.7
ndhF_p3	ndhF	3	CCATTTAGGTTTGAAATAGCGTGA	TATCCACCGAAGAAGCTCCT	24	20	37.5	50.0	57.8	57.8	0.0	0.0	4.350397	129706	131973	2554-2554	0.500000	25.2
ndhF_p4	ndhF	4	TCCATTTAGGTTTGAAATAGCGTG	TATCCACCGAAGAAGCTCCT	24	20	37.5	50.0	57.8	57.8	0.0	0.0	4.350397	129706	131973	2555-2555	0.500000	25.2
ndhF_p5	ndhF	5	CCATTTAGGTTTGAAATAGCGTGA	ACCGAAGAAGCTCCTTTTCC	24	20	37.5	50.0	57.8	57.8	0.0	0.0	4.376209	129706	131973	2549-2549	0.500000	25.0