Hotspot

rpoB

LSC · 24058-27270 · 3213 bp

Back to Clematoclethra

Pi 0.00124

MarkerSeek score 41.40

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 4
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TGGTGCCAGTATAGACCGAA	TCATTGATCAGGGCTTGACG	50.0	50.0	58.1	58.0	372 bp	1.0	79.3
rpoB_p2	TGGTGCCAGTATAGACCGAA	GGGCTTGACGGAAGAACTTT	50.0	50.0	58.1	58.4	362 bp	1.0	76.4
rpoB_p3	GGTGCCAGTATAGACCGAAA	TCATTGATCAGGGCTTGACG	50.0	50.0	57.0	58.0	371 bp	1.0	72.6
rpoB_p4	TGGTGCCAGTATAGACCGAA	CATTGATCAGGGCTTGACGG	50.0	55.0	58.1	59.0	371 bp	1.0	71.6
rpoB_p5	TGGTGCCAGTATAGACCGAA	ATCAGGGCTTGACGGAAGAA	50.0	50.0	58.1	59.0	366 bp	1.0	71.4