Hotspot

trnN-GUU-trnR-ACG

IRa · 134675-135258 · 584 bp

Back to Clematoclethra

Pi 0.00685

MarkerSeek score 62.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 584
variable_sites: 4
pi: 0.00685
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	725 bp	1.0	78.7
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	762 bp	1.0	78.1
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	676 bp	1.0	77.5
trnN-GUU-trnR-ACG_p4	GGTTGAGAACGGGAATGGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	764 bp	1.0	77.1
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	713 bp	1.0	76.9