Hotspot

trnR-ACG-trnN-GUU

IRb · 111646-112229 · 584 bp

Back to Clematoclethra

Pi 0.00685

MarkerSeek score 62.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 584
variable_sites: 4
pi: 0.00685
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	725 bp	1.0	78.7
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	762 bp	1.0	78.1
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	676 bp	1.0	77.5
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GGTTGAGAACGGGAATGGAA	55.0	50.0	57.8	57.8	764 bp	1.0	77.1
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	713 bp	1.0	76.9