Hotspot

petA-psbJ

LSC · 64925-65890 · 966 bp

Back to Clematoclethra

Pi 0.00627

MarkerSeek score 63.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 6
pi: 0.00627
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1100-1109 bp	1.0	76.7
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1101-1110 bp	1.0	76.7
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	1067-1076 bp	1.0	76.4
petA-psbJ_p4	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1068-1077 bp	1.0	74.1
petA-psbJ_p5	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1095-1104 bp	1.0	73.9