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accD-psaI

LSC · 60437-61039 · 603 bp

Back to Clematoclethra

Pi 0.00332

MarkerSeek score 60.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 603
variable_sites: 2
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAGTTCCACGATTTCTTTCCT	CCATTGCAATTGCCGGAAAT	40.9	45.0	57.6	58.0	719-728 bp	1.0	61.2
accD-psaI_p2	TCAGTTCCACGATTTCTTTCCT	AGAAACCATTGCAATTGCCG	40.9	45.0	57.6	57.9	724-733 bp	1.0	60.7
accD-psaI_p3	TCAGTTCCACGATTTCTTTCCT	GCCGGAAATACTAGGCCTAC	40.9	55.0	57.6	57.5	708-717 bp	1.0	57.8
accD-psaI_p4	TCAGTTCCACGATTTCTTTCC	CCATTGCAATTGCCGGAAAT	42.9	45.0	56.2	58.0	719-728 bp	1.0	57.5
accD-psaI_p5	CAGTTCCACGATTTCTTTCCT	CCATTGCAATTGCCGGAAAT	42.9	45.0	56.2	58.0	718-727 bp	1.0	57.4