Hotspot

ndhC-trnV-UAC

LSC · 51767-52665 · 899 bp

Back to Clematoclethra

Pi 0.00334

MarkerSeek score 60.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 3
pi: 0.00334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1033-1320 bp	1.0	78.3
ndhC-trnV-UAC_p2	CTGGTCCTTTGCTAATCGGG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.3	58.1	1050-1337 bp	1.0	76.8
ndhC-trnV-UAC_p3	CCTTTGCTAATCGGGGCTAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1045-1332 bp	1.0	76.7
ndhC-trnV-UAC_p4	TCCTTTGCTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.5	58.1	1046-1333 bp	1.0	75.4
ndhC-trnV-UAC_p5	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1038-1325 bp	1.0	74.5