Hotspot

trnK-UUU-rps16

LSC · 4327-5126 · 800 bp

Back to Clematoclethra

Pi 0.00375

MarkerSeek score 60.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 3
pi: 0.00375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	937 bp	1.0	78.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	936 bp	1.0	78.9
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.2	58.1	886 bp	1.0	78.3
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AAGGGCGGAGGTTTTTAAGG	50.0	50.0	58.2	58.1	885 bp	1.0	78.3
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GGGCGGAGGTTTTTAAGGAA	50.0	50.0	58.2	58.1	883 bp	1.0	78.2