primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps4-rps16_p1	rps4-rps16	1	GTAAGTGGGTCGGTTTGGAA	ATTCCTTCAAGTCGCACGTT	20	20	50.0	45.0	57.7	58.1	0.0	0.0	0.380313	634	1196	661-710	1.000000	89.0
rps4-rps16_p2	rps4-rps16	2	GTAAGTGGGTCGGTTTGGAA	AAGTCGCACGTTGCTTTCTA	20	20	50.0	45.0	57.7	58.1	0.0	8.1	0.398590	634	1196	653-702	1.000000	88.8
rps4-rps16_p3	rps4-rps16	3	AAGTGGGTCGGTTTGGAAAT	ATTCCTTCAAGTCGCACGTT	20	20	45.0	45.0	57.6	58.1	0.0	0.0	0.496061	634	1196	659-708	1.000000	88.0
rps4-rps16_p4	rps4-rps16	4	AAGTGGGTCGGTTTGGAAAT	AAGTCGCACGTTGCTTTCTA	20	20	45.0	45.0	57.6	58.1	0.0	8.1	0.514338	634	1196	651-700	1.000000	87.9
rps4-rps16_p5	rps4-rps16	5	AGTAAGTGGGTCGGTTTGGA	ATTCCTTCAAGTCGCACGTT	20	20	50.0	45.0	58.6	58.1	0.0	0.0	0.694296	634	1196	662-711	1.000000	86.4
rps16-trnK-UUU_p1	rps16-trnK-UUU	1	GCTCAACCTACGGAAACTGT	TCTACCAACGGTATGGACGA	20	20	50.0	50.0	57.8	57.9	0.0	10.6	0.292589	2342	2990	783-845	1.000000	89.7
rps16-trnK-UUU_p2	rps16-trnK-UUU	2	GCTCAACCTACGGAAACTGT	AAAGCCGAGTACTCTACCGT	20	20	50.0	50.0	57.8	58.2	0.0	20.3	0.333738	2342	2990	718-780	1.000000	89.3
rps16-trnK-UUU_p3	rps16-trnK-UUU	3	GCTCAACCTACGGAAACTGT	AAGCCGAGTACTCTACCGTT	20	20	50.0	50.0	57.8	58.2	0.0	20.3	0.333738	2342	2990	717-779	1.000000	89.3
rps16-trnK-UUU_p4	rps16-trnK-UUU	4	CCTTGAAAAAGGCGCTCAAC	TCTACCAACGGTATGGACGA	20	20	50.0	50.0	58.2	57.9	7.6	10.6	0.356314	2342	2990	796-858	1.000000	89.1
rps16-trnK-UUU_p5	rps16-trnK-UUU	5	CTTGAAAAAGGCGCTCAACC	TCTACCAACGGTATGGACGA	20	20	50.0	50.0	58.2	57.9	7.6	10.6	0.356314	2342	2990	795-857	1.000000	89.1
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.938078	8559	8644	174-184	1.000000	81.7
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.939466	8559	8644	175-185	1.000000	81.7
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	1.042943	8559	8644	193-203	1.000000	80.8
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CGTAATCCTGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.230929	8559	8644	174-184	1.000000	79.4
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CGTAATCCTGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.232316	8559	8644	175-185	1.000000	79.4
atpH-atpI_p1	atpH-atpI	1	TCCAGGTCCAATAGAAGCCA	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	58.0	57.9	0.0	21.3	0.100384	17385	18488	902-1275	1.000000	91.2
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	57.9	57.9	0.0	21.3	0.214244	17385	18488	875-1248	1.000000	90.3
atpH-atpI_p3	atpH-atpI	3	GATACCTTCTACGGCTTGGC	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	58.1	57.9	0.0	21.3	0.198632	17385	18488	944-1317	1.000000	90.2
atpH-atpI_p4	atpH-atpI	4	CGATACCTTCTACGGCTTGG	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	57.9	57.9	0.0	21.3	0.201463	17385	18488	945-1318	1.000000	90.2
atpH-atpI_p5	atpH-atpI	5	AACGGAAGCAGCAGAAATCA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.1	57.9	0.0	21.3	0.169579	17385	18488	857-1193	0.981250	89.8
trnS-GGA-trnV-UAC_p1	trnS-GGA-trnV-UAC	1	AGGCTTTTGTTTACCGAGGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.291646	50159	51270	964-1214	1.000000	89.7
trnS-GGA-trnV-UAC_p2	trnS-GGA-trnV-UAC	2	TTTAGGAGAGATGGCCGAGT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.358004	50159	51270	1015-1265	1.000000	89.1
trnS-GGA-trnV-UAC_p3	trnS-GGA-trnV-UAC	3	GGCTTTTGTTTACCGAGGGT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.584607	50159	51270	963-1213	1.000000	87.3
trnS-GGA-trnV-UAC_p4	trnS-GGA-trnV-UAC	4	AGGCTTTTGTTTACCGAGGG	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	0.0	0.0	0.635418	50159	51270	963-1213	1.000000	86.9
trnS-GGA-trnV-UAC_p5	trnS-GGA-trnV-UAC	5	TCTTTAGGAGAGATGGCCGA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.651619	50159	51270	1017-1267	1.000000	86.8
atpB-rbcL_p1	atpB-rbcL	1	CATCCAGTACCGGACCAATG	AGCCAACACTCGCTTTAGTC	20	20	55.0	50.0	58.0	58.2	9.5	0.0	0.249416	54361	55223	953-1037	0.975000	89.0
atpB-rbcL_p2	atpB-rbcL	2	CATCCAGTACCGGACCAATG	GCCAACACTCGCTTTAGTCT	20	20	55.0	50.0	58.0	58.2	9.5	0.0	0.249416	54361	55223	952-1036	0.975000	89.0
atpB-rbcL_p3	atpB-rbcL	3	CATCCAGTACCGGACCAATG	TGAAGCCAACACTCGCTTTA	20	20	55.0	45.0	58.0	57.8	9.5	0.0	0.294721	54361	55223	956-1040	0.975000	88.6
atpB-rbcL_p4	atpB-rbcL	4	CATCCAGTACCGGACCAATG	AAGCCAACACTCGCTTTAGT	20	20	55.0	45.0	58.0	57.7	9.5	0.0	0.302558	54361	55223	954-1038	0.975000	88.6
atpB-rbcL_p5	atpB-rbcL	5	TTCTTCAAGAGCGGAAACCC	GCCAACACTCGCTTTAGTCT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.310766	54361	55223	906-990	0.975000	88.5
ycf4-cemA_p1	ycf4-cemA	1	TGCGCGTACCAATTGAAGTA	GAAAGAAATCCACCAGGGCA	20	20	45.0	50.0	57.9	58.1	8.4	0.0	0.158370	60480	61078	687-735	0.968750	89.5
ycf4-cemA_p2	ycf4-cemA	2	AATTGAACAAAAGGCTGCGG	GAAAGAAATCCACCAGGGCA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.230011	60480	61078	722-770	0.968750	88.9
ycf4-cemA_p3	ycf4-cemA	3	AACAAAAGGCTGCGGAATTG	GAAAGAAATCCACCAGGGCA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.230011	60480	61078	717-765	0.962500	88.7
ycf4-cemA_p4	ycf4-cemA	4	TGCGCGTACCAATTGAAGTA	AGAAAGAAATCCACCAGGGC	20	20	45.0	50.0	57.9	57.8	8.4	0.0	0.303952	60480	61078	688-736	0.968750	88.3
ycf4-cemA_p5	ycf4-cemA	5	AACAAAAGGCTGCGGAATTG	AGAAAGAAATCCACCAGGGC	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.375593	60480	61078	718-766	0.962500	87.5
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	CCGCCAGTAGAAAACCGAAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.536253	64120	65499	1450-1573	1.000000	87.7
psbE-petL_p2	psbE-petL	2	ACGAATAACCAACCCGCAAT	CCGCCAGTAGAAAACCGAAA	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.655939	64120	65499	1459-1582	1.000000	86.8
psbE-petL_p3	psbE-petL	3	TGCTGACGAATAACCAACCC	CCGCCAGTAGAAAACCGAAA	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.668844	64120	65499	1464-1587	1.000000	86.6
psbE-petL_p4	psbE-petL	4	CGAATAACCAACCCGCAATG	CCGCCAGTAGAAAACCGAAA	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.672270	64120	65499	1458-1581	1.000000	86.6
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	CCGCCAGTAGAAAACCGAAA	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.722211	64120	65499	1452-1575	1.000000	86.2
petB_p1	petB	1	CTATTCTGGCAGTTCGACCG	CAAACACATGTCTTATTCTTTTCA	20	24	55.0	29.2	58.4	54.3	15.3	0.0	8.102055	74557	75987	1580-1621	1.000000	49.9
petB_p2	petB	2	GCAGTTCGACCGTGAAATTC	CAAACACATGTCTTATTCTTTTCA	20	24	50.0	29.2	57.8	54.3	0.0	0.0	7.890732	74557	75987	1572-1613	0.968750	48.6
petB_p3	petB	3	GCAGTTCGACCGTGAAATTC	CAAACACATGTCTTATTCTTTTCAA	20	25	50.0	28.0	57.8	55.0	0.0	0.0	8.180539	74557	75987	1572-1613	0.968750	48.6
petB_p4	petB	4	GCAGTTCGACCGTGAAATTC	AAACACATGTCTTATTCTTTTCA	20	23	50.0	26.1	57.8	52.8	0.0	0.0	8.387571	74557	75987	1571-1612	0.968750	48.6
petB_p5	petB	5	GCAGTTCGACCGTGAAATTC	AACACATGTCTTATTCTTTTCAA	20	23	50.0	26.1	57.8	52.8	0.0	0.0	8.387571	74557	75987	1570-1611	0.968750	48.6
ndhF_p1	ndhF	1	CCCCATCGTGGAACAATCAA	GCAACTCACTCAGTTTCCCA	20	20	50.0	50.0	58.2	58.0	5.7	0.0	0.199798	110357	112591	2839-2957	0.975000	89.4
ndhF_p2	ndhF	2	TTTGAATGGACCCCATCGTG	GCAACTCACTCAGTTTCCCA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.199798	110357	112591	2849-2967	0.975000	89.4
ndhF_p3	ndhF	3	CCCCATCGTGGAACAATCAA	AACGGACTAGTTCACAACCG	20	20	50.0	50.0	58.2	57.9	5.7	14.9	0.311599	110357	112591	2820-2938	0.962500	88.0
ndhF_p4	ndhF	4	TTTGAATGGACCCCATCGTG	AACGGACTAGTTCACAACCG	20	20	50.0	50.0	58.2	57.9	0.0	14.9	0.311599	110357	112591	2830-2948	0.962500	88.0
ndhF_p5	ndhF	5	AAGGGGTCTCTTGCTATGGA	GCAACTCACTCAGTTTCCCA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.303115	110357	112591	2944-3000	0.700000	77.6
ndhF-rpl32_p1	ndhF-rpl32	1	AAGGGGGACGAAAGGTATGA	ATCCCCATCCCACAAAAACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.087658	112592	113632	1350-1582	0.956250	89.5
ndhF-rpl32_p2	ndhF-rpl32	2	TTGGCAACCGAACCTACAAA	ATCCCCATCCCACAAAAACC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.103023	112592	113632	2021-2253	0.956250	89.4
ndhF-rpl32_p3	ndhF-rpl32	3	ACGAAAGGTATGATCCACGC	ATCCCCATCCCACAAAAACC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.112858	112592	113632	1343-1575	0.950000	89.1
ndhF-rpl32_p4	ndhF-rpl32	4	AAGGGGGACGAAAGGTATGA	CGGCATCCAGAACCCAATAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090201	112592	113632	1433-1670	0.906250	87.5
ndhF-rpl32_p5	ndhF-rpl32	5	TTGGCAACCGAACCTACAAA	CGGCATCCAGAACCCAATAA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.105566	112592	113632	2104-2341	0.906250	87.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CGATATGAACCAACGAATTCGA	AGCGTGTCTACCGATTTCAC	22	20	40.9	50.0	57.0	58.0	33.1	0.0	3.036025	113879	114662	803-861	0.950000	65.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCGATATGAACCAACGAATTCG	AGCGTGTCTACCGATTTCAC	22	20	40.9	50.0	57.0	58.0	25.6	0.0	3.036025	113879	114662	804-862	0.950000	65.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGATATGAACCAACGAATTCGA	CAGCGTGTCTACCGATTTCA	22	20	40.9	50.0	57.0	58.0	33.1	0.0	3.037396	113879	114662	804-862	0.950000	65.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCGATATGAACCAACGAATTCG	CAGCGTGTCTACCGATTTCA	22	20	40.9	50.0	57.0	58.0	25.6	0.0	3.037396	113879	114662	805-863	0.950000	65.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CGATATGAACCAACGAATTCGA	TCCTAAGAGCAGCGTGTCTA	22	20	40.9	50.0	57.0	57.9	33.1	0.0	3.152472	113879	114662	813-871	0.950000	64.8
ndhD_p1	ndhD	1	CTAATGAAGCGTGGGGATCC	GAGACAACTCGTAGCATGGG	20	20	55.0	55.0	58.1	58.1	1.9	0.0	0.187870	116113	117591	2143-2235	1.000000	90.5
ndhD_p2	ndhD	2	CTAATGAAGCGTGGGGATCC	TGCTTCTGCTCCAAGAACAG	20	20	55.0	50.0	58.1	58.1	1.9	0.0	0.227550	116113	117591	2240-2332	1.000000	90.2
ndhD_p3	ndhD	3	CTAATGAAGCGTGGGGATCC	TACGGGTTTTCGGGTCAAAG	20	20	55.0	50.0	58.1	58.1	1.9	0.0	0.234174	116113	117591	1995-2089	1.000000	90.1
ndhD_p4	ndhD	4	CTAATGAAGCGTGGGGATCC	AGAGATGATACCCTGGGACG	20	20	55.0	55.0	58.1	58.0	1.9	22.2	0.118270	116113	117591	2279-2371	0.975000	90.1
ndhD_p5	ndhD	5	GGGAGCAGTATGGGCTAATG	AGAGATGATACCCTGGGACG	20	20	55.0	55.0	57.8	58.0	0.0	22.2	0.193633	116113	117591	2293-2385	0.975000	89.5
ndhE-ndhG_p1	ndhE-ndhG	1	AAGACACATAAGCGCTCGAA	TGCTATGGCTCGTCAGTACT	20	20	45.0	50.0	57.6	58.2	12.1	1.7	0.599820	118568	118844	276-434	1.000000	87.2
ndhE-ndhG_p2	ndhE-ndhG	2	AGACACATAAGCGCTCGAAC	TGCTATGGCTCGTCAGTACT	20	20	50.0	50.0	58.4	58.2	12.1	1.7	0.605884	118568	118844	275-433	1.000000	87.2
ndhE-ndhG_p3	ndhE-ndhG	3	CAAGACACATAAGCGCTCGA	TGCTATGGCTCGTCAGTACT	20	20	50.0	50.0	58.4	58.2	12.1	1.7	0.607019	118568	118844	277-435	1.000000	87.1
ndhE-ndhG_p4	ndhE-ndhG	4	TCAAGACACATAAGCGCTCG	TGCTATGGCTCGTCAGTACT	20	20	50.0	50.0	58.4	58.2	12.1	1.7	0.607019	118568	118844	278-436	1.000000	87.1
ndhE-ndhG_p5	ndhE-ndhG	5	GCTCAAGACACATAAGCGCT	TGCTATGGCTCGTCAGTACT	20	20	50.0	50.0	58.6	58.2	2.6	1.7	0.872288	118568	118844	280-438	1.000000	85.0
ndhG_p1	ndhG	1	TCTGCTCATGAAAGAAGGCC	AACCCACACACGACCCTATA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.178424	118845	119378	1242-1405	0.987500	90.1
ndhG_p2	ndhG	2	CTGCTCATGAAAGAAGGCCA	AACCCACACACGACCCTATA	20	20	50.0	50.0	58.2	58.1	5.0	0.0	0.228344	118845	119378	1241-1404	0.987500	89.7
ndhG_p3	ndhG	3	TCTGCTCATGAAAGAAGGCC	ACCCACACACGACCCTATAT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.289813	118845	119378	1241-1404	0.987500	89.2
ndhG_p4	ndhG	4	TCTGCTCATGAAAGAAGGCC	GTCGGTTGCCAATGTCAGTA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.316467	118845	119378	1716-1877	0.987500	89.0
ndhG_p5	ndhG	5	TCTGCTCATGAAAGAAGGCC	TACATCAACCCACACACGAC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.277635	118845	119378	1248-1411	0.981250	89.0
ndhG-ndhI_p1	ndhG-ndhI	1	ACCACTCCTAGACTTCCCAC	GTCGGTTGCCAATGTCAGTA	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.256686	119379	119870	665-712	0.987500	89.4
ndhG-ndhI_p2	ndhG-ndhI	2	TCCCAACGAAAAGGCAGAAA	GTCGGTTGCCAATGTCAGTA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.256716	119379	119870	705-752	0.987500	89.4
ndhG-ndhI_p3	ndhG-ndhI	3	CCACTCCTAGACTTCCCACT	GTCGGTTGCCAATGTCAGTA	20	20	55.0	50.0	57.8	58.2	0.0	0.0	0.436347	119379	119870	664-711	0.987500	88.0
ndhG-ndhI_p4	ndhG-ndhI	4	ACCACTCCTAGACTTCCCAC	TCGGTTGCCAATGTCAGTAA	20	20	55.0	45.0	58.1	57.4	0.0	0.0	0.612619	119379	119870	664-711	0.987500	86.6
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAACGAAAAGGCAGAAA	TCGGTTGCCAATGTCAGTAA	20	20	45.0	45.0	57.9	57.4	0.0	0.0	0.612649	119379	119870	704-751	0.987500	86.6
ycf1_p1	ycf1	1	TGCTTGTTGATAACTTGGGCT	ACCGGATAATTCCAATGATGA	21	21	42.9	38.1	58.1	54.8	0.0	9.5	5.372624	126600	126719	195-210	0.925000	49.0
ycf1_p2	ycf1	2	TGCTTGTTGATAACTTGGGCT	CCGGATAATTCCAATGATGAAACA	21	24	42.9	37.5	58.1	57.6	0.0	0.0	5.514534	126600	126719	194-209	0.925000	49.0
ycf1_p3	ycf1	3	TGCTTGTTGATAACTTGGGCT	ACCGGATAATTCCAATGATGAAAC	21	24	42.9	37.5	58.1	57.6	0.0	9.5	5.515931	126600	126719	195-210	0.925000	49.0
ycf1_p4	ycf1	4	TGCTTGTTGATAACTTGGGCT	ACCGGATAATTCCAATGATGAA	21	22	42.9	36.4	58.1	55.6	0.0	9.5	5.567756	126600	126719	195-210	0.925000	49.0
ycf1_p5	ycf1	5	TGCTTGTTGATAACTTGGGC	ACCGGATAATTCCAATGATGA	20	21	45.0	38.1	56.6	54.8	0.0	9.5	5.642303	126600	126719	195-210	0.925000	49.0