Hotspot

ndhF

SSC · 101188-103407 · 2220 bp

Back to Cleistogenes

Pi 0.00115

MarkerSeek score 48.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 11
pi: 0.00115
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	58.2	58.1	2341-2353 bp	1.0	70.5
ndhF_p2	TGCATTTTGATGAGCCTCGT	GGTTTGGTGAATCGGAAACA	45.0	45.0	58.2	56.5	2340-2352 bp	1.0	67.4
ndhF_p3	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAAC	45.0	45.0	58.2	56.2	2341-2353 bp	1.0	65.0
ndhF_p4	TTGCATTTTGATGAGCCTCG	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.1	58.1	2342-2354 bp	1.0	64.5
ndhF_p5	TGCATTTTGATGAGCCTCGT	GGTTTGGTGAATCGGAAACAA	45.0	42.9	58.2	57.3	2340-2352 bp	0.909091	61.5