Hotspot

ndhF-rpl32

SSC · 103408-104327 · 920 bp

Back to Cleistogenes

Pi 0.00119
MarkerSeek score 48.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
920
variable_sites
6
pi
0.00119
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 992-1010 bp 1.0 43.0
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 993-1011 bp 1.0 43.0
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 994-1012 bp 1.0 42.9
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 995-1013 bp 1.0 42.9
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATCGAAGTACGT 55.0 35.0 58.1 53.2 996-1014 bp 1.0 42.9