Hotspot

atpI-atpH

LSC · 31423-32238 · 816 bp

Back to Cleistogenes

Pi 0.00114

MarkerSeek score 54.10

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 5
pi: 0.00114
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CTCTACAGCTTGTCCTGCAG	50.0	55.0	57.5	58.0	914-976 bp	1.0	80.2
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CTCTACAGCTTGTCCTGCAG	45.0	55.0	58.6	58.0	927-989 bp	1.0	79.7
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.5	57.9	851-913 bp	1.0	79.1
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	895-957 bp	1.0	78.8
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	828-890 bp	1.0	78.7