Hotspot

trnC-GCA-rpoB

LSC · 18374-19383 · 1010 bp

Back to Cleistogenes

Pi 0.0022

MarkerSeek score 56.70

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 10
pi: 0.0022
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	TTATTGATTAGGCGGCACCC	CCGGGTATTGTGGACATTCC	50.0	55.0	58.0	58.3	1058-1131 bp	1.0	81.9
trnC-GCA-rpoB_p2	TTATTGATTAGGCGGCACCC	CGGGTATTGTGGACATTCCC	50.0	55.0	58.0	58.3	1057-1130 bp	1.0	81.9
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1021-1094 bp	1.0	81.6
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1020-1093 bp	1.0	81.6
trnC-GCA-rpoB_p5	TTATTGATTAGGCGGCACCC	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.0	57.6	1122-1195 bp	1.0	81.5