Hotspot

psbM-petN

LSC · 16577-17364 · 788 bp

Back to Cleistogenes

Pi 0.00111

MarkerSeek score 48.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 3
pi: 0.00111
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	AGTATGGGGGAGAAGTGGAC	40.0	55.0	56.7	58.1	834-837 bp	1.0	69.2
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.7	58.1	833-836 bp	1.0	69.2
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	892-895 bp	1.0	68.9
psbM-petN_p4	AAAACAGCCAGCCAAAATGA	TATGGGGGAGAAGTGGACTC	40.0	55.0	56.7	57.8	832-835 bp	1.0	68.8
psbM-petN_p5	AAAACAGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.7	58.2	829-832 bp	1.0	68.5