Hotspot

trnG-GCC-trnfM-CAU

LSC · 11915-12270 · 356 bp

Back to Cleistogenes

Pi 0.00327

MarkerSeek score 43.30

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 5
pi: 0.00327
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GCGAGCGTAGTTCAATGGTA	AGTTTTTGGCCCTACAGAGC	50.0	50.0	58.1	58.1	518-521 bp	1.0	79.6
trnG-GCC-trnfM-CAU_p2	AATATATGCCGTAGCGAGCG	AGTTTTTGGCCCTACAGAGC	50.0	50.0	58.1	58.1	531-553 bp	1.0	79.5
trnG-GCC-trnfM-CAU_p3	GACCCAGGCGGATATATCCT	AGTTTTTGGCCCTACAGAGC	55.0	50.0	58.1	58.1	555-577 bp	1.0	79.1
trnG-GCC-trnfM-CAU_p4	CGTAGCGAGCGTAGTTCAAT	AGTTTTTGGCCCTACAGAGC	50.0	50.0	58.2	58.1	522-544 bp	1.0	78.8
trnG-GCC-trnfM-CAU_p5	TAGCGAGCGTAGTTCAATGG	AGTTTTTGGCCCTACAGAGC	50.0	50.0	57.8	58.1	520-523 bp	1.0	78.6