Hotspot

rpl16

LSC · 82946-84593 · 1648 bp

Back to Cleisostoma

Pi 0.026

MarkerSeek score 71.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1648
variable_sites: 149
pi: 0.026
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGGGCACTGCTTCTTTGATT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2149-2475 bp	1.0	89.9
rpl16_p2	TTGGGCACTGCTTCTTTGAT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2150-2476 bp	1.0	89.9
rpl16_p3	ATTGGGCACTGCTTCTTTGA	GAACACGGGGAATCGAAGAA	45.0	50.0	58.0	57.9	2374-2700 bp	1.0	89.6
rpl16_p4	TGGGCACTGCTTCTTTGATT	GAACACGGGGAATCGAAGAA	45.0	50.0	58.0	57.9	2372-2698 bp	1.0	89.6
rpl16_p5	TTGGGCACTGCTTCTTTGAT	GAACACGGGGAATCGAAGAA	45.0	50.0	58.0	57.9	2373-2699 bp	1.0	89.6