Hotspot

rps15-ycf1

SSC · 116535-116975 · 441 bp

Back to Cleisostoma

Pi 0.0435
MarkerSeek score 78.40
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
441
variable_sites
56
pi
0.0435
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AAATAAGGAACTGCGTGGCA TCGCTATTGGTTTGATGCCA 45.0 45.0 58.1 57.9 1125-1201 bp 0.909091 85.4
rps15-ycf1_p2 AAATAAGGAACTGCGTGGCA TGATGCCAGTAACAGCAGTC 45.0 50.0 58.1 57.9 1113-1189 bp 0.818182 81.2
rps15-ycf1_p3 CCAAAGGTCCGCAAATAGGA TGATGCCAGTAACAGCAGTC 50.0 50.0 57.9 57.9 1202-1254 bp 0.636364 72.4
rps15-ycf1_p4 TCCAAAGGTCCGCAAATAGG TGATGCCAGTAACAGCAGTC 50.0 50.0 57.9 57.9 1203-1255 bp 0.636364 72.4
rps15-ycf1_p5 CCTATTTCCAAAGGTCCGCA TGATGCCAGTAACAGCAGTC 50.0 50.0 57.9 57.9 1209-1246 bp 0.363636 58.9