Hotspot

rpl32-trnL-UAG

SSC · 111141-111651 · 511 bp

Back to Cleisostoma

Pi 0.0463

MarkerSeek score 83.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 511
variable_sites: 78
pi: 0.0463
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGTCAAAAAGTTTCTTTG	TCCTAAGAGCAGCGTGTCTA	40.9	50.0	56.9	57.9	598-727 bp	0.909091	60.5
rpl32-trnL-UAG_p2	GGGCAGTCAAAAAGTTTCTTTG	AGCAGCGTGTCTACCAATTT	40.9	45.0	56.9	57.8	591-720 bp	0.909091	60.1
rpl32-trnL-UAG_p3	GGGCAGTCAAAAAGTTTCTTTG	CTAAGAGCAGCGTGTCTACC	40.9	55.0	56.9	57.8	596-725 bp	0.909091	60.0
rpl32-trnL-UAG_p4	GGGCAGTCAAAAAGTTTCTTTG	CCTAAGAGCAGCGTGTCTAC	40.9	55.0	56.9	57.8	597-726 bp	0.909091	59.9
rpl32-trnL-UAG_p5	GGGCAGTCAAAAAGTTTCTTTG	GAGCAGCGTGTCTACCAATT	40.9	50.0	56.9	58.3	592-721 bp	0.909091	59.3