Hotspot

psbB-psbT

LSC · 75322-75697 · 376 bp

Back to Cleisostoma

Pi 0.0502

MarkerSeek score 84.30

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 59
pi: 0.0502
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	509-611 bp	1.0	78.7
psbB-psbT_p2	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGA	45.0	47.6	57.7	57.3	508-610 bp	1.0	76.5
psbB-psbT_p3	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGAA	45.0	45.5	57.7	58.0	508-610 bp	1.0	73.8
psbB-psbT_p4	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	471-561 bp	0.909091	72.0
psbB-psbT_p5	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	470-560 bp	0.909091	69.8