Hotspot

ndhC-trnV-UAC

LSC · 50846-51580 · 735 bp

Back to Cleisostoma

Pi 0.038

MarkerSeek score 77.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 72
pi: 0.038
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGGAATGCCCAGAAAATCTCA	AGAAGGTCTACGGTTCGAGT	42.9	50.0	57.3	57.8	631-804 bp	0.909091	72.1
ndhC-trnV-UAC_p2	AGGAATGCCCAGAAAATCTCA	GAAGGTCTACGGTTCGAGTC	42.9	55.0	57.3	57.5	630-803 bp	0.909091	69.3
ndhC-trnV-UAC_p3	AGGAATGCCCAGAAAATCTCA	AGCCCAATGCAACAAAATGC	42.9	45.0	57.3	58.5	713-886 bp	0.909091	69.2
ndhC-trnV-UAC_p4	AGGAATGCCCAGAAAATCTCA	GTTCGAGTCCGTATAGCCCT	42.9	55.0	57.3	58.7	619-792 bp	0.909091	68.3
ndhC-trnV-UAC_p5	AGGAATGCCCAGAAAATCTCAT	AGAAGGTCTACGGTTCGAGT	40.9	50.0	57.7	57.8	631-804 bp	0.909091	67.9