Hotspot

trnT-GGU-psbD

LSC · 33011-34024 · 1014 bp

Back to Cleisostoma

Pi 0.0193

MarkerSeek score 78.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 83
pi: 0.0193
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1096-1206 bp	1.0	87.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1099-1209 bp	1.0	87.3
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.3	57.7	1073-1183 bp	1.0	85.9
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CGGACCAGCCTACAAAAACA	55.0	50.0	58.1	58.4	1176 bp	0.090909	39.9
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CGGACCAGCCTACAAAAACA	50.0	50.0	58.1	58.4	1179 bp	0.090909	39.7