Hotspot

trnC-GCA-petN

LSC · 28656-29479 · 824 bp

Back to Cleisostoma

Pi 0.0219

MarkerSeek score 77.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 70
pi: 0.0219
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGCAAATCCTTTATCCCCGG	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	845-969 bp	1.0	89.4
trnC-GCA-petN_p2	GCAAATCCTTTATCCCCGGT	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	844-968 bp	1.0	89.4
trnC-GCA-petN_p3	GATGTAATTTGGCGGCATGG	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	886-1010 bp	1.0	88.5
trnC-GCA-petN_p4	GGATGTAATTTGGCGGCATG	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	887-1011 bp	1.0	88.5
trnC-GCA-petN_p5	ATCCTTTATCCCCGGTTCGA	GCTCTAAAGCCCACTCCTTC	50.0	55.0	58.2	58.0	840-964 bp	1.0	88.3