Hotspot

rpoB-trnC-GCA

LSC · 26951-28584 · 1634 bp

Back to Cleisostoma

Pi 0.0321

MarkerSeek score 80.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1634
variable_sites: 155
pi: 0.0321
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAATTCTTCCGTCAAGCCCT	CCGGGGATAAAGGATTTGCA	45.0	50.0	57.4	57.9	1570-1812 bp	1.0	86.2
rpoB-trnC-GCA_p2	AAATTCTTCCGTCAAGCCCT	ACCGGGGATAAAGGATTTGC	45.0	50.0	57.4	57.9	1571-1813 bp	1.0	86.2
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTT	CCGGGGATAAAGGATTTGCA	45.0	50.0	57.4	57.9	1789 bp	0.090909	38.4
rpoB-trnC-GCA_p4	AATTCTTCCGTCAAGCCCTT	ACCGGGGATAAAGGATTTGC	45.0	50.0	57.4	57.9	1790 bp	0.090909	38.4
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTT	TCGAACCGGGGATAAAGGAT	45.0	50.0	57.4	58.2	1794 bp	0.090909	37.3