Hotspot

trnS-GCU-trnG-GCC

LSC · 8433-9651 · 1219 bp

Back to Cleisostoma

Pi 0.0393
MarkerSeek score 82.10
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1219
variable_sites
161
pi
0.0393
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1175-1288 bp 1.0 63.7
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1174-1287 bp 1.0 63.7
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1178-1291 bp 1.0 62.6
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1177-1290 bp 1.0 62.6
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1176-1289 bp 1.0 62.6