Hotspot

ycf1

IRb · 108849-111707 · 2859 bp

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Pi 0.0604

MarkerSeek score 74.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 2859
variable_sites: 170
pi: 0.0604
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGAAAGTTGCAAAATCATCA	TCGATGGAATTACAAATGGAGT	33.3	36.4	54.5	55.5	2952 bp	0.5	20.0
ycf1_p2	TGGAAAGTTGCAAAATCATCA	CGATGGAATTACAAATGGAGTAGG	33.3	41.7	54.5	57.5	2951 bp	0.5	20.0
ycf1_p3	TGGAAAGTTGCAAAATCATCA	CATCGATGGAATTACAAATGGAGT	33.3	37.5	54.5	57.4	2954 bp	0.5	20.0
ycf1_p4	TGGAAAGTTGCAAAATCATCA	TCATCGATGGAATTACAAATGGAG	33.3	37.5	54.5	57.1	2955 bp	0.5	20.0
ycf1_p5	TGGAAAGTTGCAAAATCATCAT	TCGATGGAATTACAAATGGAGT	31.8	36.4	55.1	55.5	2952 bp	0.5	20.0