Hotspot

clpP

LSC · 69036-71111 · 2076 bp

Back to Clausia

Pi 0.249

MarkerSeek score 69.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2076
variable_sites: 188
pi: 0.249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.36
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGAGTACATGTTCCTCGTCG	ATACGCAATGGGGGTGAATC	55.0	50.0	58.2	58.0	1419-2655 bp	1.0	90.3
clpP_p2	CCGAGTACATGTTCCTCGTC	ATACGCAATGGGGGTGAATC	55.0	50.0	57.8	58.0	1420-2656 bp	1.0	90.3
clpP_p3	TGACATTTCGGATTGGCTGT	GAACAGAAAGCAAACGACCG	45.0	50.0	57.8	58.0	1633-2869 bp	1.0	89.7
clpP_p4	CGAGTACATGTTCCTCGTCG	GAACAGAAAGCAAACGACCG	55.0	50.0	58.2	58.0	1685-2921 bp	1.0	89.6
clpP_p5	CCGAGTACATGTTCCTCGTC	GAACAGAAAGCAAACGACCG	55.0	50.0	57.8	58.0	1686-2922 bp	1.0	89.6