Hotspot

accD

LSC · 56213-57676 · 1464 bp

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Pi 0.0802

MarkerSeek score 76.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1464
variable_sites: 117
pi: 0.0802
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCATGTAAATATAGGCCAGAAGCT	AGGGTTTTCGTTTAATTCTGA	37.5	33.3	57.6	53.3	1524-1962 bp	1.0	45.7
accD_p2	TGTAAATATAGGCCAGAAGCTCT	AGGGTTTTCGTTTAATTCTGA	39.1	33.3	56.8	53.3	1521 bp	0.5	20.0
accD_p3	TGTAAATATAGGCCAGAAGCTCT	AAGGGTTTTCGTTTAATTCTGA	39.1	31.8	56.8	54.2	1522 bp	0.5	20.0
accD_p4	TGTAAATATAGGCCAGAAGCTCT	AGGGTTTTCGTTTAATTCTGAT	39.1	31.8	56.8	54.0	1521 bp	0.5	20.0
accD_p5	AAATATAGGCCAGAAGCTCT	AGGGTTTTCGTTTAATTCTGA	40.0	33.3	53.5	53.3	1518 bp	0.5	20.0