Hotspot

rps15-ycf1

SSC · 124557-127117 · 2561 bp

Back to Clausia

Pi 0.073

MarkerSeek score 78.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 2561
variable_sites: 181
pi: 0.073
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	ACGGGGTGGACAAAATCTTC	50.0	50.0	58.0	58.1	2834 bp	0.5	59.2
rps15-ycf1_p2	CAGCAGTCGTTGACGTTTTC	CGGGGTGGACAAAATCTTCA	50.0	50.0	58.0	58.1	2833 bp	0.5	59.2
rps15-ycf1_p3	CAGCAGTCGTTGACGTTTTC	TACACGGGGTGGACAAAATC	50.0	50.0	58.0	57.8	2837 bp	0.5	58.5
rps15-ycf1_p4	ACAAATAAGCCAGCAGTCGT	ACGGGGTGGACAAAATCTTC	45.0	50.0	57.8	58.1	2844 bp	0.5	57.8
rps15-ycf1_p5	ACAAATAAGCCAGCAGTCGT	CGGGGTGGACAAAATCTTCA	45.0	50.0	57.8	58.1	2843 bp	0.5	57.8