Hotspot

psaJ-rpl33

LSC · 66143-66567 · 425 bp

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Pi 0.0463

MarkerSeek score 75.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 19
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CATATCTTTCCGTAGCGCCA	AACTTCTTTACCCTTGGCCA	50.0	45.0	57.9	56.9	559-561 bp	1.0	75.2
psaJ-rpl33_p2	ACATATCTTTCCGTAGCGCC	AACTTCTTTACCCTTGGCCA	50.0	45.0	57.8	56.9	560-562 bp	1.0	75.1
psaJ-rpl33_p3	CATATCTTTCCGTAGCGCCA	GAACTTCTTTACCCTTGGCCA	50.0	47.6	57.9	58.1	560-562 bp	1.0	74.6
psaJ-rpl33_p4	ACATATCTTTCCGTAGCGCC	GAACTTCTTTACCCTTGGCCA	50.0	47.6	57.8	58.1	561-563 bp	1.0	74.6
psaJ-rpl33_p5	CATATCTTTCCGTAGCGCCA	TCGAACTTCTTTACCCTTGGC	50.0	47.6	57.9	58.2	562-564 bp	1.0	73.8