Hotspot

petA-psbJ

LSC · 61729-62740 · 1012 bp

Back to Clausia

Pi 0.0379

MarkerSeek score 77.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 37
pi: 0.0379
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTCCAAGGCCTTTTGTTCT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.5	58.1	1173-1196 bp	1.0	80.2
petA-psbJ_p2	GTGTCCAAGGCCTTTTGTTC	GCTGGTATTCCTGTGATCGG	50.0	55.0	57.5	58.1	1174-1197 bp	1.0	79.8
petA-psbJ_p3	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.4	58.1	1150-1173 bp	1.0	79.4
petA-psbJ_p4	AGGATCGGTTGTTTTGGCAC	GCTGGTATTCCTGTGATCGG	50.0	55.0	59.0	58.1	1149-1172 bp	1.0	75.6
petA-psbJ_p5	TTAGGATCGGTTGTTTTGGCA	GCTGGTATTCCTGTGATCGG	42.9	55.0	58.1	58.1	1151-1174 bp	1.0	74.8