Hotspot

psbC-trnS-UCA

LSC · 34139-34323 · 185 bp

Back to Clausia

Pi 0.0444

MarkerSeek score 76.40

Primer available yes

Label psbC-trnS-UCA

Feature Metadata

label_name: psbC-trnS-UCA
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 8
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UCA_p1	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	315-318 bp	1.0	79.8
psbC-trnS-UCA_p2	TCGTGGGTCATTTATGGCAC	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	365-368 bp	1.0	79.6
psbC-trnS-UCA_p3	CGGCAGCAGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	276-279 bp	1.0	78.8
psbC-trnS-UCA_p4	CGGCAGCAGGATTTGAAAAA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.6	58.2	326-329 bp	1.0	78.5
psbC-trnS-UCA_p5	TTCGTGGGTCATTTATGGCA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.5	58.2	366-369 bp	1.0	77.6