primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	trnH-CAC-psbA	1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	57.9	58.1	0.0	12.9	0.188044	77	320	302-303	1.000000	87.5
trnH-CAC-psbA_p2	trnH-CAC-psbA	2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	58.2	58.1	0.0	12.9	0.213831	77	320	303-304	1.000000	87.3
trnH-CAC-psbA_p3	trnH-CAC-psbA	3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	77	320	317-318	1.000000	87.0
trnH-CAC-psbA_p4	trnH-CAC-psbA	4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	77	320	320-321	1.000000	86.8
trnH-CAC-psbA_p5	trnH-CAC-psbA	5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	58.2	57.9	0.0	13.4	0.226475	77	320	318-319	1.000000	86.7
trnS-AGC-trnG-GGA_p1	trnS-AGC-trnG-GGA	1	CCAACGCTTTAGTCCACTCA	TACCACTAAACTATACCCGC	20	20	50.0	45.0	57.8	54.0	0.0	0.0	4.174894	7684	8264	627-632	1.000000	53.2
trnS-AGC-trnG-GGA_p2	trnS-AGC-trnG-GGA	2	AACGCTTTAGTCCACTCAGC	TACCACTAAACTATACCCGC	20	20	50.0	45.0	58.2	54.0	2.0	0.0	4.214052	7684	8264	625-630	1.000000	52.9
trnS-AGC-trnG-GGA_p3	trnS-AGC-trnG-GGA	3	CCAACGCTTTAGTCCACTCA	TTACCACTAAACTATACCCGC	20	21	50.0	42.9	57.8	54.9	0.0	0.0	4.311154	7684	8264	628-633	1.000000	52.1
trnS-AGC-trnG-GGA_p4	trnS-AGC-trnG-GGA	4	AACGCTTTAGTCCACTCAGC	TTACCACTAAACTATACCCGC	20	21	50.0	42.9	58.2	54.9	2.0	0.0	4.350311	7684	8264	626-631	1.000000	51.8
trnS-AGC-trnG-GGA_p5	trnS-AGC-trnG-GGA	5	GCTTTAGTCCACTCAGCCAT	TACCACTAAACTATACCCGC	20	20	50.0	45.0	57.6	54.0	0.9	0.0	4.421025	7684	8264	622-627	1.000000	51.3
psbC-trnS-UCA_p1	psbC-trnS-UCA	1	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.3	58.2	0.0	17.2	0.471697	34139	34323	315-318	1.000000	79.8
psbC-trnS-UCA_p2	psbC-trnS-UCA	2	TCGTGGGTCATTTATGGCAC	GATGGCTCCGGTCTTGAAAA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.433551	34139	34323	365-368	1.000000	79.6
psbC-trnS-UCA_p3	psbC-trnS-UCA	3	CGGCAGCAGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	20	20	45.0	55.0	57.6	58.2	0.0	17.2	0.654128	34139	34323	276-279	1.000000	78.8
psbC-trnS-UCA_p4	psbC-trnS-UCA	4	CGGCAGCAGGATTTGAAAAA	GATGGCTCCGGTCTTGAAAA	20	20	45.0	50.0	57.6	58.2	0.0	0.0	0.615983	34139	34323	326-329	1.000000	78.5
psbC-trnS-UCA_p5	psbC-trnS-UCA	5	TTCGTGGGTCATTTATGGCA	GATGGCTCCGGTCTTGAAAA	20	20	45.0	50.0	57.5	58.2	0.0	0.0	0.677428	34139	34323	366-369	1.000000	77.6
trnF-UUC-ndhJ_p1	trnF-UUC-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.062041	47079	47680	732-741	1.000000	84.0
trnF-UUC-ndhJ_p2	trnF-UUC-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	47079	47680	705-714	1.000000	83.9
trnF-UUC-ndhJ_p3	trnF-UUC-ndhJ	3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	47079	47680	704-713	1.000000	83.9
trnF-UUC-ndhJ_p4	trnF-UUC-ndhJ	4	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAACGGATCTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.132182	47079	47680	729-738	1.000000	83.4
trnF-UUC-ndhJ_p5	trnF-UUC-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGATAGCCATCCACGACTGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.276511	47079	47680	739-748	1.000000	82.2
ndhC-trnV-GUA_p1	ndhC-trnV-GUA	1	TGCCAAAACAGGAATAGCACT	TCGAACTGTTTTACCGAGCA	21	20	42.9	45.0	58.1	57.5	0.0	0.0	1.637189	49402	50277	993-997	1.000000	71.0
ndhC-trnV-GUA_p2	ndhC-trnV-GUA	2	TGCCAAAACAGGAATAGCACT	GTTCGAGTCCGTATAGCCCT	21	20	42.9	55.0	58.1	58.7	0.0	0.0	1.808053	49402	50277	965-969	1.000000	69.8
ndhC-trnV-GUA_p3	ndhC-trnV-GUA	3	TGCCAAAACAGGAATAGCAC	TCGAACTGTTTTACCGAGCA	20	20	45.0	45.0	56.6	57.5	0.0	0.0	1.906868	49402	50277	993-997	1.000000	68.8
ndhC-trnV-GUA_p4	ndhC-trnV-GUA	4	TGCCAAAACAGGAATAGCACT	CGAACTGTTTTACCGAGCAG	21	20	42.9	50.0	58.1	57.1	0.0	0.0	1.980795	49402	50277	992-996	1.000000	68.3
ndhC-trnV-GUA_p5	ndhC-trnV-GUA	5	TGCCAAAACAGGAATAGCACT	TTCGAGTCCGTATAGCCCTA	21	20	42.9	50.0	58.1	57.1	0.0	0.0	2.061623	49402	50277	964-968	1.000000	67.7
accD_p1	accD	1	TCATGTAAATATAGGCCAGAAGCT	AGGGTTTTCGTTTAATTCTGA	24	21	37.5	33.3	57.6	53.3	5.0	0.0	10.090670	56213	57676	1524-1962	1.000000	45.7
accD_p2	accD	2	TGTAAATATAGGCCAGAAGCTCT	AGGGTTTTCGTTTAATTCTGA	23	21	39.1	33.3	56.8	53.3	5.0	0.0	9.804849	56213	57676	1521-1521	0.500000	20.0
accD_p3	accD	3	TGTAAATATAGGCCAGAAGCTCT	AAGGGTTTTCGTTTAATTCTGA	23	22	39.1	31.8	56.8	54.2	5.0	0.0	9.943548	56213	57676	1522-1522	0.500000	20.0
accD_p4	accD	4	TGTAAATATAGGCCAGAAGCTCT	AGGGTTTTCGTTTAATTCTGAT	23	22	39.1	31.8	56.8	54.0	5.0	0.0	10.163933	56213	57676	1521-1521	0.500000	20.0
accD_p5	accD	5	AAATATAGGCCAGAAGCTCT	AGGGTTTTCGTTTAATTCTGA	20	21	40.0	33.3	53.5	53.3	5.0	0.0	10.193370	56213	57676	1518-1518	0.500000	20.0
petA-psbJ_p1	petA-psbJ	1	TGTCCAAGGCCTTTTGTTCT	GCTGGTATTCCTGTGATCGG	20	20	45.0	55.0	57.5	58.1	17.6	0.0	0.581520	61729	62740	1173-1196	1.000000	80.2
petA-psbJ_p2	petA-psbJ	2	GTGTCCAAGGCCTTTTGTTC	GCTGGTATTCCTGTGATCGG	20	20	50.0	55.0	57.5	58.1	17.6	0.0	0.637972	61729	62740	1174-1197	1.000000	79.8
petA-psbJ_p3	petA-psbJ	3	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCCTGTGATCGG	20	20	45.0	55.0	57.4	58.1	0.0	0.0	0.699335	61729	62740	1150-1173	1.000000	79.4
petA-psbJ_p4	petA-psbJ	4	AGGATCGGTTGTTTTGGCAC	GCTGGTATTCCTGTGATCGG	20	20	50.0	55.0	59.0	58.1	0.0	0.0	1.170098	61729	62740	1149-1172	1.000000	75.6
petA-psbJ_p5	petA-psbJ	5	TTAGGATCGGTTGTTTTGGCA	GCTGGTATTCCTGTGATCGG	21	20	42.9	55.0	58.1	58.1	0.0	0.0	1.263841	61729	62740	1151-1174	1.000000	74.8
psaJ-rpl33_p1	psaJ-rpl33	1	CATATCTTTCCGTAGCGCCA	AACTTCTTTACCCTTGGCCA	20	20	50.0	45.0	57.9	56.9	2.3	0.0	1.212650	66143	66567	559-561	1.000000	75.2
psaJ-rpl33_p2	psaJ-rpl33	2	ACATATCTTTCCGTAGCGCC	AACTTCTTTACCCTTGGCCA	20	20	50.0	45.0	57.8	56.9	2.3	0.0	1.214132	66143	66567	560-562	1.000000	75.1
psaJ-rpl33_p3	psaJ-rpl33	3	CATATCTTTCCGTAGCGCCA	GAACTTCTTTACCCTTGGCCA	20	21	50.0	47.6	57.9	58.1	2.3	0.0	1.277463	66143	66567	560-562	1.000000	74.6
psaJ-rpl33_p4	psaJ-rpl33	4	ACATATCTTTCCGTAGCGCC	GAACTTCTTTACCCTTGGCCA	20	21	50.0	47.6	57.8	58.1	2.3	0.0	1.278944	66143	66567	561-563	1.000000	74.6
psaJ-rpl33_p5	psaJ-rpl33	5	CATATCTTTCCGTAGCGCCA	TCGAACTTCTTTACCCTTGGC	20	21	50.0	47.6	57.9	58.2	2.3	0.0	1.374228	66143	66567	562-564	1.000000	73.8
clpP_p1	clpP	1	CGAGTACATGTTCCTCGTCG	ATACGCAATGGGGGTGAATC	20	20	55.0	50.0	58.2	58.0	26.8	1.6	0.207315	69036	71111	1419-2655	1.000000	90.3
clpP_p2	clpP	2	CCGAGTACATGTTCCTCGTC	ATACGCAATGGGGGTGAATC	20	20	55.0	50.0	57.8	58.0	29.8	1.6	0.209068	69036	71111	1420-2656	1.000000	90.3
clpP_p3	clpP	3	TGACATTTCGGATTGGCTGT	GAACAGAAAGCAAACGACCG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.217544	69036	71111	1633-2869	1.000000	89.7
clpP_p4	clpP	4	CGAGTACATGTTCCTCGTCG	GAACAGAAAGCAAACGACCG	20	20	55.0	50.0	58.2	58.0	26.8	0.0	0.207289	69036	71111	1685-2921	1.000000	89.6
clpP_p5	clpP	5	CCGAGTACATGTTCCTCGTC	GAACAGAAAGCAAACGACCG	20	20	55.0	50.0	57.8	58.0	29.8	0.0	0.209042	69036	71111	1686-2922	1.000000	89.6
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.013783	85642	85729	719-725	1.000000	82.4
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	TCCAGCGACGGTTTCATTAG	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.013783	85642	85729	720-726	1.000000	82.4
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	CACTTGACTGTCTACGGCTC	CCAGCGACGGTTTCATTAGA	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.020610	85642	85729	1288-1301	1.000000	82.4
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	CCCCTCTATGCCTTACGGTA	CCAGCGACGGTTTCATTAGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.009953	85642	85729	1382-1395	1.000000	82.3
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	CCCCTCTATGCCTTACGGTA	TCCAGCGACGGTTTCATTAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.009953	85642	85729	1383-1396	1.000000	82.3
ycf1_p1	ycf1	1	TGGAAAGTTGCAAAATCATCA	TCGATGGAATTACAAATGGAGT	21	22	33.3	36.4	54.5	55.5	0.0	0.0	8.936162	108849	111707	2952-2952	0.500000	20.0
ycf1_p2	ycf1	2	TGGAAAGTTGCAAAATCATCA	CGATGGAATTACAAATGGAGTAGG	21	24	33.3	41.7	54.5	57.5	0.0	0.0	9.000853	108849	111707	2951-2951	0.500000	20.0
ycf1_p3	ycf1	3	TGGAAAGTTGCAAAATCATCA	CATCGATGGAATTACAAATGGAGT	21	24	33.3	37.5	54.5	57.4	0.0	18.0	9.079701	108849	111707	2954-2954	0.500000	20.0
ycf1_p4	ycf1	4	TGGAAAGTTGCAAAATCATCA	TCATCGATGGAATTACAAATGGAG	21	24	33.3	37.5	54.5	57.1	0.0	24.6	9.321359	108849	111707	2955-2955	0.500000	20.0
ycf1_p5	ycf1	5	TGGAAAGTTGCAAAATCATCAT	TCGATGGAATTACAAATGGAGT	22	22	31.8	36.4	55.1	55.5	0.0	0.0	9.347242	108849	111707	2952-2952	0.500000	20.0
rpl32-trnL-CUA_p1	rpl32-trnL-CUA	1	GCACTCTCATACGGGTTTCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.504361	114740	115287	982-982	0.500000	56.0
rpl32-trnL-CUA_p2	rpl32-trnL-CUA	2	GCACTCTCATACGGGTTTCA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	57.6	57.8	0.0	0.0	0.565088	114740	115287	975-975	0.500000	55.5
rpl32-trnL-CUA_p3	rpl32-trnL-CUA	3	TGAGCACTCTCATACGGGTT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.567178	114740	115287	985-985	0.500000	55.5
rpl32-trnL-CUA_p4	rpl32-trnL-CUA	4	GCACTCTCATACGGGTTTCA	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	57.6	57.8	0.0	0.0	0.576379	114740	115287	981-981	0.500000	55.4
rpl32-trnL-CUA_p5	rpl32-trnL-CUA	5	GCACTCTCATACGGGTTTCA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	57.6	57.8	0.0	0.0	0.576379	114740	115287	980-980	0.500000	55.4
rps15-ycf1_p1	rps15-ycf1	1	CAGCAGTCGTTGACGTTTTC	ACGGGGTGGACAAAATCTTC	20	20	50.0	50.0	58.0	58.1	3.2	0.0	0.099551	124557	127117	2834-2834	0.500000	59.2
rps15-ycf1_p2	rps15-ycf1	2	CAGCAGTCGTTGACGTTTTC	CGGGGTGGACAAAATCTTCA	20	20	50.0	50.0	58.0	58.1	3.2	0.0	0.100902	124557	127117	2833-2833	0.500000	59.2
rps15-ycf1_p3	rps15-ycf1	3	CAGCAGTCGTTGACGTTTTC	TACACGGGGTGGACAAAATC	20	20	50.0	50.0	58.0	57.8	3.2	0.0	0.193250	124557	127117	2837-2837	0.500000	58.5
rps15-ycf1_p4	rps15-ycf1	4	ACAAATAAGCCAGCAGTCGT	ACGGGGTGGACAAAATCTTC	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.279367	124557	127117	2844-2844	0.500000	57.8
rps15-ycf1_p5	rps15-ycf1	5	ACAAATAAGCCAGCAGTCGT	CGGGGTGGACAAAATCTTCA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.280717	124557	127117	2843-2843	0.500000	57.8
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	CCAGCGACGGTTTCATTAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.013783	153345	153432	719-725	1.000000	82.4
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	TCCAGCGACGGTTTCATTAG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.013783	153345	153432	720-726	1.000000	82.4
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CCAGCGACGGTTTCATTAGA	CCCCTCTATGCCTTACGGTA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.009953	153345	153432	1395-1395	0.500000	59.9
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	TCCAGCGACGGTTTCATTAG	CCCCTCTATGCCTTACGGTA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.009953	153345	153432	1396-1396	0.500000	59.9
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CCAGCGACGGTTTCATTAGA	CACTTGACTGTCTACGGCTC	20	20	50.0	55.0	58.0	58.0	0.0	9.5	0.020610	153345	153432	1301-1301	0.500000	59.8