Hotspot

ycf1

SSC · 128243-132580 · 4338 bp

Back to Clausena

Pi 0.026

MarkerSeek score 59.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4338
variable_sites: 262
pi: 0.026
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACGAAATGCATGTTCCCCT	ACGGACCTTATCGTGGAAGA	45.0	50.0	58.1	58.2	423-429 bp	1.0	87.3
ycf1_p2	GAATGGGCAGTTGCTTGAAC	ACGGACCTTATCGTGGAAGA	50.0	50.0	57.9	58.2	595-601 bp	1.0	86.1
ycf1_p3	TCAGGCGGGAAATGTCAAAA	ACGGACCTTATCGTGGAAGA	45.0	50.0	58.0	58.2	657-663 bp	1.0	85.8
ycf1_p4	TGGTATCTTTCGCCTTTGGG	ACGGACCTTATCGTGGAAGA	50.0	50.0	57.9	58.2	781-787 bp	0.6	66.7
ycf1_p5	AAAAAGAGGGGAATGGGCAG	ACGGACCTTATCGTGGAAGA	50.0	50.0	58.1	58.2	611 bp	0.2	46.3