Hotspot

rps3

LSC · 86458-87117 · 660 bp

Back to Clausena

Pi 0.0145

MarkerSeek score 54.90

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 660
variable_sites: 24
pi: 0.0145
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	AGTCGCACACTAAGCATAGC	TCCCGGGCATCTACCATTAT	50.0	50.0	58.1	58.0	1517-1736 bp	1.0	84.1
rps3_p2	AGTCGCACACTAAGCATAGC	TCAATTTCCGTGGACATGCA	50.0	45.0	58.1	58.1	1387-1606 bp	1.0	84.1
rps3_p3	AGTCGCACACTAAGCATAGC	AGGGCGGAGTTATCTGATCA	50.0	50.0	58.1	57.9	1009-1025 bp	0.8	75.1
rps3_p4	AGTCGCACACTAAGCATAGC	GCAGCAGCAAATGGTATTGG	50.0	50.0	58.1	58.1	1122 bp	0.2	46.9
rps3_p5	TCAGTTTTATTGGCTCGGGG	TCCCGGGCATCTACCATTAT	50.0	50.0	57.9	58.0	1938 bp	0.2	46.7