Hotspot

petA-psbJ

LSC · 66482-67582 · 1101 bp

Back to Clausena

Pi 0.0305

MarkerSeek score 74.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 75
pi: 0.0305
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	CCGGATTAGGTTCGTCCTTG	42.9	55.0	57.2	58.1	1150-1180 bp	1.0	72.5
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	TCCGGATTAGGTTCGTCCTT	42.9	50.0	57.2	58.1	1151-1181 bp	1.0	71.9
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGAA	CCGGATTAGGTTCGTCCTTG	40.9	55.0	57.9	58.1	1150-1180 bp	1.0	70.0
petA-psbJ_p4	TTGAGAAGGTTCAATTGTCCGA	CCGGATTAGGTTCGTCCTTG	40.9	55.0	57.9	58.1	1151-1181 bp	1.0	70.0
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGAA	TCCGGATTAGGTTCGTCCTT	40.9	50.0	57.9	58.1	1151-1181 bp	1.0	69.4