Hotspot

ndhK-ndhC

LSC · 53257-53322 · 66 bp

Back to Clausena

Pi 0.0364

MarkerSeek score 72.30

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 6
pi: 0.0364
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AAGCTGGAGAGTCTTGACCA	AGGAGCATTAGAGTGGTCTT	50.0	45.0	58.3	55.5	184-193 bp	1.0	62.8
ndhK-ndhC_p2	AGCTGGAGAGTCTTGACCAA	AAGGAGCATTAGAGTGGTCT	50.0	45.0	58.3	55.5	184-193 bp	1.0	62.8
ndhK-ndhC_p3	AGCTGGAGAGTCTTGACCAA	AGGAGCATTAGAGTGGTCTT	50.0	45.0	58.3	55.5	183-192 bp	1.0	62.8
ndhK-ndhC_p4	AAGCTGGAGAGTCTTGACCA	AAGGAGCATTAGAGTGGTCT	50.0	45.0	58.3	55.5	185-194 bp	1.0	62.7
ndhK-ndhC_p5	AGCTGGAGAGTCTTGACCAA	AAGGAGCATTAGAGTGGTCTT	50.0	42.9	58.3	56.3	184-193 bp	1.0	61.2