Hotspot

trnG-GCC-trnM-CAU

LSC · 39522-39698 · 177 bp

Back to Clausena

Pi 0.0354

MarkerSeek score 73.00

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 177
variable_sites: 15
pi: 0.0354
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	251-276 bp	1.0	87.0
trnG-GCC-trnM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	287-312 bp	1.0	86.0
trnG-GCC-trnM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	249-274 bp	1.0	85.9
trnG-GCC-trnM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	271-296 bp	1.0	84.9
trnG-GCC-trnM-CAU_p5	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	272-297 bp	1.0	84.8