Hotspot

trnD-GUC-trnY-GUA

LSC · 32712-33197 · 486 bp

Back to Clausena

Pi 0.0384

MarkerSeek score 81.80

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 486
variable_sites: 39
pi: 0.0384
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	539-559 bp	1.0	83.9
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	538-558 bp	1.0	83.9
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	560-580 bp	1.0	82.8
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	559-579 bp	1.0	82.6
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	TTATTCTTCCTGGCCTGGGT	50.0	50.0	58.5	58.3	616 bp	0.2	41.6