Hotspot

atpF-atpH

LSC · 14031-14094 · 64 bp

Back to Clausena

Pi 0.0516

MarkerSeek score 78.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 8
pi: 0.0516
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GTGCTGTGTCTGGACTTCAA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	695-1134 bp	1.0	82.3
atpF-atpH_p2	GCCGGTTAACCAAAGAAACG	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	341-778 bp	1.0	81.3
atpF-atpH_p3	TGGGTCCAGCCTATTCTTGA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	479-916 bp	1.0	81.0
atpF-atpH_p4	CAGCATAAAAGGGGGTTGGT	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.1	58.0	919-1332 bp	0.8	74.8
atpF-atpH_p5	TGGGTCCAGCCTATTCTTGA	AAATCCTGTGTTGTGCCTGT	50.0	45.0	58.0	57.9	706-1106 bp	0.6	64.0