Hotspot

trnS-CGA-trnR-UCU

LSC · 10580-10768 · 189 bp

Back to Clausena

Pi 0.07

MarkerSeek score 79.20

Primer available yes

Label trnS-CGA-trnR-UCU

Feature Metadata

label_name: trnS-CGA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 25
pi: 0.07
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-CGA-trnR-UCU_p1	ACGATGCGGGTTCGATTC	AGAAGACCTCTGTCCTATCCA	55.6	47.6	57.9	56.9	244-258 bp	1.0	58.8
trnS-CGA-trnR-UCU_p2	ACGATGCGGGTTCGATTC	AGGTTTAGAAGACCTCTGTCCT	55.6	45.5	57.9	57.9	250-264 bp	1.0	57.9
trnS-CGA-trnR-UCU_p3	GATGCGGGTTCGATTCCC	AGAAGACCTCTGTCCTATCCA	61.1	47.6	58.3	56.9	242-256 bp	1.0	57.4
trnS-CGA-trnR-UCU_p4	GATGCGGGTTCGATTCCC	AGGTTTAGAAGACCTCTGTCCT	61.1	45.5	58.3	57.9	248-262 bp	1.0	56.7
trnS-CGA-trnR-UCU_p5	GTTCGATTCCCGCTACCC	AGAAGACCTCTGTCCTATCCA	61.1	47.6	57.6	56.9	235-249 bp	1.0	56.1