Hotspot

psbK-psbI

LSC · 8156-8568 · 413 bp

Back to Clausena

Pi 0.0415

MarkerSeek score 73.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 413
variable_sites: 32
pi: 0.0415
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	AGAGGGTTGCTCAAAAAGGG	TGAGTGGATTAAAGCGTCGG	50.0	50.0	58.0	58.0	1371-1396 bp	1.0	86.5
psbK-psbI_p2	GAGGGTTGCTCAAAAAGGGA	TGAGTGGATTAAAGCGTCGG	50.0	50.0	58.0	58.0	1370-1395 bp	1.0	86.5
psbK-psbI_p3	AGAGGGTTGCTCAAAAAGGG	AGGCCCTGTTGAACGAAATT	50.0	45.0	58.0	58.0	1726-1762 bp	0.8	78.1
psbK-psbI_p4	AGAGGGTTGCTCAAAAAGGG	AAGGCCCTGTTGAACGAAAT	50.0	45.0	58.0	58.0	1727-1763 bp	0.8	78.0
psbK-psbI_p5	GAGGGTTGCTCAAAAAGGGA	AGGCCCTGTTGAACGAAATT	50.0	45.0	58.0	58.0	1725-1761 bp	0.8	78.0