Hotspot

rps16-trnQ-UUG

LSC · 6266-7546 · 1281 bp

Back to Clausena

Pi 0.0286

MarkerSeek score 74.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1281
variable_sites: 85
pi: 0.0286
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1325-1372 bp	1.0	88.2
rps16-trnQ-UUG_p2	GCGGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1323-1370 bp	1.0	88.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1307-1354 bp	1.0	87.4
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1318-1365 bp	1.0	86.8
rps16-trnQ-UUG_p5	AGCGGATCATGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1324-1371 bp	1.0	85.8