Hotspot

trnK-UUU-rps16

LSC · 4523-5086 · 564 bp

Back to Clausena

Pi 0.0361

MarkerSeek score 73.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 44
pi: 0.0361
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGTACTTAAAAGCCGAG	GGTGTGTTGCTGCCATTTTT	50.0	45.0	58.0	58.0	1237-1409 bp	1.0	88.7
trnK-UUU-rps16_p2	CGGTCTTACAAACTCTCCCG	GGTGTGTTGCTGCCATTTTT	55.0	45.0	58.0	58.0	1304-1476 bp	1.0	88.5
trnK-UUU-rps16_p3	AGCCGTACTTAAAAGCCGAG	TGCCTAAGGATTTTCGGACG	50.0	50.0	58.0	58.0	1119 bp	0.2	47.8
trnK-UUU-rps16_p4	CGGTCTTACAAACTCTCCCG	TGCCTAAGGATTTTCGGACG	55.0	50.0	58.0	58.0	1186 bp	0.2	47.8
trnK-UUU-rps16_p5	AATTGGAACCCTAGGGCTCT	TGCCTAAGGATTTTCGGACG	50.0	50.0	58.0	58.0	1368 bp	0.2	47.7