Hotspot

rpl32-trnL-UAG

SSC · 102816-103399 · 584 bp

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Pi 0.0134
MarkerSeek score 67.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
584
variable_sites
15
pi
0.0134
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAAAATCTGTTTCAACTGGGA AGCGTGTCTACCGATTTCAC 40.9 50.0 57.7 58.0 676-682 bp 1.0 65.0
rpl32-trnL-UAG_p2 GCAAAATCTGTTTCAACTGGGA CAGCGTGTCTACCGATTTCA 40.9 50.0 57.7 58.0 677-683 bp 1.0 65.0
rpl32-trnL-UAG_p3 AGCAAAATCTGTTTCAACTGGG AGCGTGTCTACCGATTTCAC 40.9 50.0 57.7 58.0 677-683 bp 1.0 65.0
rpl32-trnL-UAG_p4 AGCAAAATCTGTTTCAACTGGG CAGCGTGTCTACCGATTTCA 40.9 50.0 57.7 58.0 678-684 bp 1.0 65.0
rpl32-trnL-UAG_p5 GCAAAATCTGTTTCAACTGGGA TCCTAAGAGCAGCGTGTCTA 40.9 50.0 57.7 57.9 686-692 bp 1.0 64.0