Hotspot

ycf4-cemA

LSC · 56765-57369 · 605 bp

Back to Cladopus

Pi 0.016

MarkerSeek score 60.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 605
variable_sites: 19
pi: 0.016
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAAATTGAGAAAAGGGCGGC	ATCGACTCTATCTGATCCAT	50.0	40.0	58.0	52.4	686-694 bp	1.0	42.6
ycf4-cemA_p2	CGGCGGAATTGGCTTATTTT	ATCGACTCTATCTGATCCAT	45.0	40.0	57.4	52.4	670-678 bp	1.0	42.6
ycf4-cemA_p3	GAAATTGAGAAAAGGGCGGC	TATCGACTCTATCTGATCCAT	50.0	38.1	58.0	52.5	687-695 bp	1.0	42.6
ycf4-cemA_p4	GAAATTGAGAAAAGGGCGGC	TTATCGACTCTATCTGATCCA	50.0	38.1	58.0	52.7	688-696 bp	1.0	42.5
ycf4-cemA_p5	GAAATTGAGAAAAGGGCGGC	TTTATCGACTCTATCTGATCCA	50.0	36.4	58.0	53.6	689-697 bp	1.0	42.5